D2HGDH Polyklonaler Antikörper

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• KD/KO Validated

D2HGDH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 13895-1-AP

Synonyme

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13895-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13895-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13895-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 13895-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13895-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13895-1-AP (D2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver tissue using 13895-1-AP

  IP result of anti-D2HGDH (13895-1-AP for IP and Detection) with mouse liver tissue lysate.

• 

  IP experiment of mouse liver using 13895-1-AP

  IP result of anti-D2HGDH (IP:13895-1-AP, 3ug; Detection:13895-1-AP 1:500) with mouse liver tissue lysate 5000ug.

• 

  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13895-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13895-1-AP (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13895-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13895-1-AP (D2HGDH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, humanes Herzgewebe, humanes Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13895-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA D2HGDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: D2HGDH fusion protein Ag4857
• Vollständiger Name: D-2-hydroxyglutarate dehydrogenase
• Berechnetes Molekulargewicht: 521 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC036604
• Gene symbol: D2HGDH
• Gene ID (NCBI): 728294
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Metab; Hypoxia Induces Production of L-2-Hydroxyglutarate.; Authors - Andrew M Intlekofer ; KD Validated
human	WB	Cell Metab; Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.; Authors - William M Oldham ; KD Validated
human	WB, IF	Nat Commun; D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.; Authors - An-Ping Lin
human	WB	Cell Chem Biol; MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.; Authors - ZhiJun Qiu ; KO Validated
human	WB	Leukemia; MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.; Authors - An-Ping Lin
	WB	Oncotarget; D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.; Authors - Shenghong Ma