Cytokeratin 8-specific Polyklonaler Antikörper

Cytokeratin 8-specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27105-1-AP

Synonyme

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytokeratin 8-specific, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

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Galerie der Validierungsdaten

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  WB analysis using 27105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 27105-1-AP (Cytokeratin 8-specific antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 27105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27105-1-AP (Cytokeratin 8 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 27105-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 8-specific antibody (27105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolongewebe, Maushautgewebe, Rattenhautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

27105-1-AP bindet in WB, IHC, IF, ELISA Cytokeratin 8-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 8-specific fusion protein Ag25844
• Vollständiger Name: keratin 8
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC008200
• Gene symbol: KRT8
• Gene ID (NCBI): 3856
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

Publikationen

Species	Application	Title
human	WB	Transl Lung Cancer Res; Identification of lysosomal genes associated with prognosis in lung adenocarcinoma; Authors - Houqiang Li
human	IHC	Environ Toxicol; Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer; Authors - Hua Chen