Cytokeratin 18 Monoklonaler Antikörper

Cytokeratin 18 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

IF, FC (Intra), FC

Konjugation

FITC Fluorescent Dye

Publikationen

1

CloneNo.

1G11C4

Kat-Nr. : FITC-66187

Synonyme

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

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Galerie der Validierungsdaten

• 

  IF Staining of HeLa using FITC-66187

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FITC Cytokeratin 18 antibody (FITC-66187, Clone: 1G11C4 ) at dilution of 1:200.

• 

  FC experiment of HeLa using FITC-66187

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug FITC Anti-Human Cytokeratin 18 (FITC-66187, Clone:1G11C4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

FITC-66187 bindet in IF, FC (Intra), FC Cytokeratin 18 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: Rind
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cytokeratin 18 fusion protein Ag1260
• Vollständiger Name: keratin 18
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000180
• Gene symbol: KRT18
• Gene ID (NCBI): 3875
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 494 nm / 520 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.

Publikationen

Species	Application	Title
bovine	FC	J Dairy Sci; Role of sortilin 1 (SORT1) on lipid metabolism in bovine liver; Authors - Wei Yang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C