Cystatin C Rekombinanter Antikörper

Cystatin C Rekombinant Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1I20

Kat-Nr. : 82441-1-RR

Synonyme

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin

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Galerie der Validierungsdaten

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  WB analysis using 82441-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 82441-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 82441-1-RR

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, human milk, human urine
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:15000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82441-1-RR bindet in WB, IHC, ELISA Cystatin C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Cystatin C fusion protein Ag2890
• Vollständiger Name: cystatin C
• Berechnetes Molekulargewicht: 146 aa, 16 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC013083
• Gene symbol: CST3
• Gene ID (NCBI): 1471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.