Cyclin B2 Monoklonaler Antikörper

Cyclin B2 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G8B7

Kat-Nr. : CL647-67726

Synonyme

CCNB2, cyclin B2, G2/mitotic specific cyclin B2, HsT17299

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Galerie der Validierungsdaten

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  IF Staining of mouse testis using CL647-67726

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 647 Cyclin B2 antibody (CL647-67726, Clone: 2G8B7 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-67726 bindet in IF Cyclin B2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cyclin B2 fusion protein Ag16329
• Vollständiger Name: cyclin B2
• Berechnetes Molekulargewicht: 398 aa, 45 kDa
• GenBank-Zugangsnummer: BC105086
• Gene symbol: CCNB2
• Gene ID (NCBI): 9133
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of human cancers, such as adrenocortical carcinoma, breast carcinoma, colorectal adenocarcinoma, pituitary adenoma and gastric cancer. The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status.