Cardiac Troponin T Monoklonaler Antikörper

Cardiac Troponin T Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

4E2D8

Kat-Nr. : 68300-1-Ig

Synonyme

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle

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Galerie der Validierungsdaten

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  WB analysis of pig heart using 68300-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit heart using 68300-1-Ig

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 68300-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 68300-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of chicken heart using 68300-1-Ig

  chicken heart tissue were subjected to SDS PAGE followed by western blot with 68300-1-Ig (cTnT antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 68300-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 68300-1-Ig (Cardiac Troponin T antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of H9C2 using 68300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 68300-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using cTnT antibody (68300-1-Ig, Clone: 4E2D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, Hausschwein-Herzgewebe, Kaninchenherzgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe, H9C2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68300-1-Ig bindet in WB, IHC, IF, ELISA Cardiac Troponin T und zeigt Reaktivität mit Hausschwein, Huhn, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Vollständiger Name: troponin T type 2 (cardiac)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC002653
• Gene symbol: TNNT2
• Gene ID (NCBI): 7139
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.