CYP3A5 Polyklonaler Antikörper

CYP3A5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13737-1-AP

Synonyme

CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13737-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

13737-1-AP bindet in WB, IHC, ELISA CYP3A5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP3A5 fusion protein Ag4674
• Vollständiger Name: cytochrome P450, family 3, subfamily A, polypeptide 5
• Berechnetes Molekulargewicht: 502 aa, 57 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC033862
• Gene symbol: CYP3A5
• Gene ID (NCBI): 1577
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
		Oncotarget; Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.; Authors - Liuqin He
human	WB	Int J Mol Sci; Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion; Authors - Rocchina Miglionico