CYP2J2 Polyklonaler Antikörper

CYP2J2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13562-1-AP

Synonyme

Arachidonic acid epoxygenase, CPJ2, CYP2J2, CYPIIJ2, Cytochrome P450 2J2

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Galerie der Validierungsdaten

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  WB analysis using 13562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13562-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13562-1-AP (CYP2J2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13562-1-AP (CYP2J2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13562-1-AP (CYP2J2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HepG2-Zellen, L02-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe, humanes Leberkarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13562-1-AP bindet in WB, IF, IHC, ELISA CYP2J2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP2J2 fusion protein Ag4435
• Vollständiger Name: cytochrome P450, family 2, subfamily J, polypeptide 2
• Berechnetes Molekulargewicht: 502 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58-65 kDa
• GenBank-Zugangsnummer: BC032594
• Gene symbol: CYP2J2
• Gene ID (NCBI): 1573
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2J2(Cytochrome P450 2J2) belongs to the cytochrome P450 family. This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. The human CYP2J member, CYP2J2, is shown to be expressed in several tissues such as the small intestine, liver, heart, kidney, and lung and may play a role in the oxidative bioactivation of arachidonic acid(PMID:12386130).

Publikationen

Species	Application	Title
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
rat	WB,IHC,IF	Life Sci; CYP2J2-produced epoxyeicosatrienoic acids attenuate ischemia/reperfusion-induced acute kidney injury by activating the SIRT1-FoxO3a pathway.; Authors - Ye Zhu
human	WB	Pharmacology; Berberine Induces CYP2J2 Expression in Human U251 Glioma Cells via Regulation of Peroxisome Proliferator-Activated Receptor Alpha.; Authors - Xuming Yu
		PeerJ; CYP2C11 played a significant role in down-regulating rat blood pressure under the challenge of a high-salt diet.; Authors - Wei Liu
human	WB	Placenta; Altered distribution of fatty acid exerting lipid metabolism and transport at the maternal-fetal interface in fetal growth restriction; Authors - Zhongmei Yang