CYP11A1 Polyklonaler Antikörper

CYP11A1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

46

Kat-Nr. : 13363-1-AP

Synonyme

Cholesterol desmolase, CYP11A, CYP11A1, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), P450SCC

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Galerie der Validierungsdaten

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  WB analysis using 13363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13363-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HeLa-Zellen, Maus-Eierstockgewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Produktinformation

13363-1-AP bindet in WB, IF, IHC, ELISA CYP11A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP11A1 fusion protein Ag4182
• Vollständiger Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 521 aa, 60 kDa
• Beobachtetes Molekulargewicht: 49-52 kDa
• GenBank-Zugangsnummer: BC032329
• Gene symbol: CYP11A1
• Gene ID (NCBI): 1583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP11A1 is also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. It is the first enzyme of all steroidogenic pathways, its elimination results in syndromes of steroid deficiency, including electrolyte imbalance, aberrant gene regulation, and suppression of male sex organ development(PMID: 12145347). Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al. The murine 32-kDa isoform of Cyp11a1 is not produced by omission of the first ATG-containing exon (as is the case for human CYP11A1) but perhaps by alternative translational initiation(PMID:19342447).

Publikationen

Species	Application	Title
rat	WB	Science; Pregnenolone can protect the brain from cannabis intoxication.; Authors - Monique Vallée
human	WB	Nat Commun; 1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.; Authors - Michaela Schedel
mouse	WB	Autophagy; Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.; Authors - Wenwen Liu
human	WB,IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
rat	WB	Biomed Pharmacother; Caffeic acid's role in mitigating polycystic ovary syndrome by countering apoptosis and ER stress triggered by oxidative stress; Authors - Yi-Fen Chiang