CYP11A1 Polyklonaler Antikörper

CYP11A1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13363-1-AP

Synonyme

Cholesterol desmolase, CYP11A, CYP11A1, CYPXIA1, Cytochrome P450 11A1, Cytochrome P450(scc), P450SCC



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plazenta-Gewebe, HeLa-Zellen, Maus-Eierstockgewebe, Maushodengewebe, Rattenhodengewebe
Erfolgreiche Detektion in IHChumanes Plazenta-Gewebe, humanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13363-1-AP bindet in WB, IF, IHC, ELISA CYP11A1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CYP11A1 fusion protein Ag4182
Vollständiger Name cytochrome P450, family 11, subfamily A, polypeptide 1
Berechnetes Molekulargewicht 521 aa, 60 kDa
Beobachtetes Molekulargewicht 49-52 kDa
GenBank-ZugangsnummerBC032329
Gene symbol CYP11A1
Gene ID (NCBI) 1583
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP11A1 is also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. It is the first enzyme of all steroidogenic pathways, its elimination results in syndromes of steroid deficiency, including electrolyte imbalance, aberrant gene regulation, and suppression of male sex organ development(PMID: 12145347). Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al. The murine 32-kDa isoform of Cyp11a1 is not produced by omission of the first ATG-containing exon (as is the case for human CYP11A1) but perhaps by alternative translational initiation(PMID:19342447).

Protokolle

Produktspezifische Protokolle
WB protocol for CYP11A1 antibody 13363-1-APProtokoll herunterladen
IHC protocol for CYP11A1 antibody 13363-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratWB

Science

Pregnenolone can protect the brain from cannabis intoxication.

Authors - Monique Vallée
humanWB

Nat Commun

1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.

Authors - Michaela Schedel
mouseWB

Autophagy

Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.

Authors - Wenwen Liu
humanWB,IHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
mouseWB

Part Fibre Toxicol

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Authors - Haibo Jin
ratWB

Biomed Pharmacother

Caffeic acid's role in mitigating polycystic ovary syndrome by countering apoptosis and ER stress triggered by oxidative stress

Authors - Yi-Fen Chiang