CYB5R3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYB5R3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Kat-Nr. : 10894-1-AP

Synonyme

NADH-cytochrome b5 reductase 3, EC:1.6.2.2, Diaphorase-1, Diaphorase 1, DIA1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10894-1-AP

  WB results of CYB5R3 antibody (10894-1-AP, 1:4000) with si-Control and si-CYB5R3 transfected HeLa cells.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10894-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10894-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10894-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 10894-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10894-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10894-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP (CYB5R3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10894-1-AP

  IP result of anti-CYB5R3 (IP:10894-1-AP, 3ug; Detection:10894-1-AP 1:1000) with HepG2 cells lysate 6000ug.

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  IHC staining of human normal colon using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10894-1-AP (CYB5R3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 10894-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10894-1-AP (CYB5R3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 10894-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYB5R3 antibody (10894-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: human normal colon, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Produktinformation

10894-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CYB5R3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYB5R3 fusion protein Ag1339
• Vollständiger Name: cytochrome b5 reductase 3
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC004821
• Gene symbol: CYB5R3
• Gene ID (NCBI): 1727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.

Publikationen

Species	Application	Title
mouse	WB	Sci Transl Med; Cyb5r3-based mechanism and reversal of secondary failure to sulfonylurea in diabetes; Authors - Hitoshi Watanabe ; KO Validated
		J Cell Biol; Dia1-dependent adhesions are required by epithelial tissues to initiate invasion.; Authors - Tim B Fessenden
human,mouse	WB,IF	JCI Insight; CYB5R3 in type II alveolar epithelial cells protects against lung fibrosis by suppressing TGF-β1 signaling; Authors - Marta Bueno
rat	IHC	Brain Struct Funct; High expression of cytochrome b 5 reductase isoform 3/cytochrome b 5 system in the cerebellum and pyramidal neurons of adult rat brain.; Authors - A K Samhan-Arias
mouse	IHC	Blood Adv; Smooth muscle cytochrome b5 reductase 3 deficiency accelerates pulmonary hypertension development in sickle cell mice.; Authors - Katherine C Wood
mouse	WB	Free Radic Biol Med; Sex-specific metabolic adaptations in transgenic mice overexpressing cytochrome b5 reductase-3; Authors - Luz Marina Sánchez-Mendoza