CXCL8/IL-8 Polyklonaler Antikörper

CXCL8/IL-8 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 17038-1-AP

Synonyme

C X C motif chemokine 8, CXCL8, CXCL8/IL-8, Emoctakin, GCP 1, GCP/IL 8 protein I, GCP/IL 8 protein II, GCP/IL 8 protein III, GCP/IL 8 protein IV, GCP/IL 8 protein V, GCP/IL 8 protein VI, GCP1, IL 8, IL8, IL-8, interleukin 8, LYNAP, MDNCF

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Galerie der Validierungsdaten

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  WB analysis using 17038-1-AP

  PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PMA, LPS and Brefeldin A treated THP-1 cells
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

17038-1-AP bindet in WB, IHC, IF, ELISA CXCL8/IL-8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL8/IL-8 fusion protein Ag10552
• Vollständiger Name: interleukin 8
• Berechnetes Molekulargewicht: 99 aa, 11 kDa
• GenBank-Zugangsnummer: BC013615
• Gene symbol: IL8
• Gene ID (NCBI): 3576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Publikationen

Species	Application	Title
human		Mol Cancer; RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.; Authors - Han-Hee Park
human		EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	IHC,IF	Cell Death Discov; Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.; Authors - Guojing Li
human		Cell Death Dis; Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.; Authors - Kezhou Zhu
	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
human	IHC	J Neurooncol; Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.; Authors - Pedram Tabatabaei