CUL4A Polyklonaler Antikörper

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• KD/KO Validated

CUL4A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10693-1-AP

Synonyme

CUL 4A, CUL4A, cullin 4A, Cullin-4A

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 10693-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10693-1-AP (CUL4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10693-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10693-1-AP (CUL4A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10693-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10693-1-AP (CUL4A antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10693-1-AP bindet in WB, IF, IHC, ELISA CUL4A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL4A fusion protein Ag1030
• Vollständiger Name: cullin 4A
• Berechnetes Molekulargewicht: 77 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC008308
• Gene symbol: CUL4A
• Gene ID (NCBI): 8451
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2.

Publikationen

Species	Application	Title
human	IHC	Breast Cancer Res; Comprehensive characterization of the DNA amplification at 13q34 in human breast cancer reveals TFDP1 and CUL4A as likely candidate target genes.; Authors - Melchor Lorenzo L
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated
human	WB	Mol Carcinog; Norcantharidin promotes cancer radiosensitization through Cullin1 neddylation-mediated CDC6 protein degradation.; Authors - Tanggang Deng ; KD Validated
mouse	WB,IF	Sci Adv; Stress-induced reduction of Na+/H+ exchanger isoform 1 promotes maladaptation of neuroplasticity and exacerbates depressive behaviors; Authors - Ye Li
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated