CUGBP2 Polyklonaler Antikörper

CUGBP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 12921-1-AP

Synonyme

CELF-2, CELF2, CELF 2, Bruno-like protein 3, BRUNOL3

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Galerie der Validierungsdaten

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  WB analysis using 12921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 12921-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12921-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12921-1-AP (CUGBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 12921-1-AP

  IP result of anti-CUGBP2 (IP:12921-1-AP, 3ug; Detection:12921-1-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.

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  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12921-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12921-1-AP (CUGBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12921-1-AP (CUGBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen, Maus-Thymusgewebe, MCF-7-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12921-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CUGBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUGBP2 fusion protein Ag3594
• Vollständiger Name: CUG triplet repeat, RNA binding protein 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036391
• Gene symbol: CUGBP2
• Gene ID (NCBI): 10659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CELF (CUGBP and ETR-3 Like Factor)/Bruno-like protein family plays important roles in the regulation of alternative splicing and translation. CUGBP2 is a member of this family of proteins that possesses several alternatively spliced exons. CUGBP2 induces smooth muscle-specific exon inclusion via binding to uridine purine repeat elements (URE) in ACTN1. Although CUGBP2 is known as a splicing activator for the N-methyl-D-aspartate receptor 1 (NMDA R1) exon 21, CUGBP2 represses NMDA R1 exon 5 inclusion. In addition, CUGBP2 was reported to repress the inclusion of the Ir exon 11.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Inhibition of COX2 enhances the chemosensitivity of dichloroacetate in cervical cancer cells.; Authors - Bo Li
mouse	IHC	Brain Res; Transcriptional induction and translational inhibition of Arc and Cugbp2 in mice hippocampus after transient global ischemia under normothermic condition.; Authors - Otsuka Noboru N
human	WB	Exp Biol Med (Maywood); Hypoxic colorectal cancer-secreted exosomes deliver miR-210-3p to normoxic tumor cells to elicit a protumoral effect.; Authors - Liuqing Ge
human	WB,IF	Pharmacol Res; Exosomal miR-625-3p secreted by cancer-associated fibroblasts in colorectal cancer promotes EMT and chemotherapeutic resistance by blocking the CELF2/WWOX pathway; Authors - Ying Zhang