CUEDC2 Polyklonaler Antikörper

CUEDC2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20123-1-AP

Synonyme

C10orf66, CUE domain containing 2, CUEDC2

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Galerie der Validierungsdaten

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  WB analysis using 20123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20123-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 20123-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20123-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20123-1-AP (CUEDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20123-1-AP

  IP Result of anti-CUEDC2 (IP:20123-1-AP, 4ug; Detection:20123-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human ovary tumor using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

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  IHC staining of human malignant melanoma using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens)..

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  IHC staining of human breast cancer using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20123-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20123-1-AP (CUEDC2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 20123-1-AP (CUEDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Nierengewebe, Jurkat-Zellen, Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes malignes Melanomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20123-1-AP bindet in WB, IP, IHC, IF, ELISA CUEDC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUEDC2 fusion protein Ag13847
• Vollständiger Name: CUE domain containing 2
• Berechnetes Molekulargewicht: 287 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000262
• Gene symbol: CUEDC2
• Gene ID (NCBI): 79004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.