Cathepsin L Polyklonaler Antikörper

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• KD/KO Validated

Cathepsin L Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10938-1-AP

Synonyme

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, Major excreted protein, MEP

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Galerie der Validierungsdaten

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  WB analysis of A549 using 10938-1-AP

  WB result of Cathepsin L antibody (10938-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin L transfected A549 cells.

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  WB analysis of A549 using 10938-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10938-1-AP (Cathepsin L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 10938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10938-1-AP (Cathepsin L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 10938-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 10938-1-AP (Cathepsin L Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10938-1-AP (Cathepsin L antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

10938-1-AP bindet in WB, IF, IHC, ELISA Cathepsin L und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin L fusion protein Ag1373
• Vollständiger Name: cathepsin L1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 36-39 kDa, 29 kDa
• GenBank-Zugangsnummer: BC012612
• Gene symbol: Cathepsin L
• Gene ID (NCBI): 1514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Publikationen

Species	Application	Title
human		Emerg Microbes Infect; GILT restricts the cellular entry mediated by the envelope glycoproteins of SARS-CoV, Ebola virus and Lassa fever virus.; Authors - Danying Chen
human	IF	Front Microbiol; Cronobacter sakazakii ATCC 29544 Translocated Human Brain Microvascular Endothelial Cells via Endocytosis, Apoptosis Induction, and Disruption of Tight Junction.; Authors - Tong Jin
human	WB	J Cancer Res Clin Oncol; Expressions and significances of CTSL, the target of COVID-19 on GBM.; Authors - Qiang Dong
human	WB	Cancer Cell Int; Differential secretome of pancreatic cancer cells in serum-containing conditioned medium reveals CCT8 as a new biomarker of pancreatic cancer invasion and metastasis.; Authors - Peng Liu
human	IHC	Exp Eye Res; A proteomic approach towards understanding the pathogenesis of Mooren's ulcer.; Authors - Hao Chi
human	IHC	BMC Infect Dis; COVID-19: a fatal case of acute liver failure associated with SARS-CoV-2 infection in pre-existing liver cirrhosis.; Authors - Jana Ihlow