Cathepsin K Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cathepsin K Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

74

Rezensionen (2)

Kat-Nr. : 11239-1-AP

Synonyme

CTSK, EC:3.4.22.38, CTSO2, CTSO, catK

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Galerie der Validierungsdaten

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  WB analysis using 11239-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11239-1-AP (Cathepsin K antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of ROS1728 using 11239-1-AP

  IP result of anti-Cathepsin K (IP:11239-1-AP, 3ug; Detection:11239-1-AP 1:500) with ROS1728 cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

• 

  IHC staining of human osteosarcoma using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:100.

• 

  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11239-1-AP (Cathepsin K antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11239-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11239-1-AP (Cathepsin K antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse brain using 11239-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody (11239-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11239-1-AP

  Immunofluorescent analysis of HepG2 cells using 11239-1-AP (Cathepsin K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, ROS1728-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Osteosarkomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 10 publications below
• IF: See 13 publications below

Produktinformation

11239-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Cathepsin K und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin K fusion protein Ag1761
• Vollständiger Name: cathepsin K
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 27 kDa, 38~46 kDa
• GenBank-Zugangsnummer: BC016058
• Gene symbol: Cathepsin K
• Gene ID (NCBI): 1513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSK(cathepsin K), also named CTSO, CTSO2, is a recently identified lysosomal cysteine proteinase. CTSK is synthesized as a proenzyme of 38 kDa and subsequently enters acidic lysosomal compartments, in which the propeptide is cleaved and transformed into an active enzyme and it may influence adipocyte differentiation through modifying extracellular matrix components(PMID:16912123). It is revealed both the 46-kDa cathepsin-K precursor and the 30-kDa mature form in mouse bone extracts (PMID:9811821). The high CTSK protein levels are only detected in primary cultured fibroblasts derived from normal and neoplastic breast tissue, where the 37- and 25-kDa bands to be detected correspond to pro- and mature proteins through western blot (PMID:18765527). The full length protein has a signal peptides with 15 amino acids and a propeptide with 99 amino acids. CTSK may have cross reaction with the other members of cathepsin family and form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Publikationen

Species	Application	Title
		Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
mouse	IHC,IF	Biomaterials; BMP2 immune complexes promote new bone formation by facilitating the direct contact between osteoclasts and osteoblasts.; Authors - Yamei Xu
mouse		ACS Appl Mater Interfaces; Nel-like Molecule Type 1 Combined with Gold Nanoparticles Modulates Macrophage Polarization, Osteoclastogenesis, and Oral Microbiota in Periodontitis; Authors - Wenzheng Liao
mouse		Br J Pharmacol; Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis.; Authors - Tongling Huang
mouse	IHC	Arthritis Rheum; Osteoarthritic change is delayed in a Ctsk-knockout mouse model of osteoarthritis.; Authors - Kozawa Eiji E
mouse	IHC	Arthritis Rheum; Co-opting endogenous immunoglobulin for the regulation of inflammation and osteoclastogenesis in humans and mice.; Authors - MacLellan Lindsay M LM

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabel (Verified Customer) (07-03-2020)

Antibody worked really well to detect Cathepsin K in protein from mouse osteoclasts. Blot was really clean with no other bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary mouse osteoclasts

Cathepsin K Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:11239-1-AP)

FH

RICHA (Verified Customer) (10-09-2019)

Good but hard to get the signal

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

Cathepsin K Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:11239-1-AP)