Cathepsin F Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cathepsin F Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11055-1-AP

Synonyme

Cathepsin F, CATSF, CTSF

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Galerie der Validierungsdaten

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  IHC staining of human prostate cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11055-1-AP (Cathepsin F antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse liver using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11055-1-AP (Cathepsin F antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11055-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11055-1-AP (Cathepsin F antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Herzgewebe, humanes Magenkrebsgewebe, Mausherzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Produktinformation

11055-1-AP bindet in WB, IHC, ELISA Cathepsin F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin F fusion protein Ag1521
• Vollständiger Name: cathepsin F
• Berechnetes Molekulargewicht: 53 kDa and 34 kDa
• GenBank-Zugangsnummer: BC011682
• Gene symbol: Cathepsin F
• Gene ID (NCBI): 8722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSF(Cathepsin F) is a member of the papain family of cysteine proteases. These enzymes represent a major component of the lysosomal proteolytic system. They are synthesized as inactive precursors consisting of a signal sequence, a propeptide, and a catalytically active mature region. Cathepsins are routed to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The deduced 484-amino acid cathepsin F protein has a signal sequence and potential glycosylation sites.

Publikationen

Species	Application	Title
rat	IHC	Stem Cell Res Ther; Adipose-derived stem cells alleviate radiation-induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression.; Authors - Chaoling Yao ; KD Validated
human	IHC	Brain Res Bull; Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.; Authors - Saghizadeh Mehrnoosh M
human	IHC	Proc Natl Acad Sci U S A; A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB; Authors - Yuqi Lin
human	WB	Oncol Res; Cathepsin F Knockdown Induces Proliferation and Inhibits Apoptosis in Gastric Cancer Cells; Authors - Ce Ji ; KD Validated