CTPS2 Polyklonaler Antikörper

CTPS2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12852-1-AP

Synonyme

CTP synthase 2, CTP synthase II, CTP synthetase 2, CTPS2, UTP ammonia ligase 2

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12852-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12852-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 12852-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12852-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12852-1-AP (CTPS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12852-1-AP

  IP result of anti-CTPS2 (IP:12852-1-AP, 4ug; Detection:12852-1-AP 1:300) with HeLa cells lysate 1085 ug.

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 12852-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12852-1-AP (CTPS2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, humanes Nebennierengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

12852-1-AP bindet in WB, IP, IF, IHC, ELISA CTPS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTPS2 fusion protein Ag3490
• Vollständiger Name: CTP synthase II
• Berechnetes Molekulargewicht: 586 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC034986
• Gene symbol: CTPS2
• Gene ID (NCBI): 56474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTPS2 (CTP synthase 2) is also named as UTP--ammonia ligase 2 and belongs to the CTP synthase family.It catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen and constitutes the rate-limiting enzyme in the synthesis of cytosine nucleotides.

Publikationen

Species	Application	Title
human	IF	Int Rev Cell Mol Biol; Molecular cell biology and immunobiology of mammalian rod/ring structures.; Authors - Wendy C Carcamo
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise
human	WB	J Genet Genomics; Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.; Authors - Gerson Dierley Keppeke