CTNS Polyklonaler Antikörper

CTNS Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13085-1-AP

Synonyme

Cystinosin

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Galerie der Validierungsdaten

• 

  WB analysis using 13085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13085-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13085-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13085-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13085-1-AP (CTNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CTNS antibody (13085-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 13085-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 13085-1-AP (CTNS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

13085-1-AP bindet in WB, IF/ICC, ELISA CTNS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTNS fusion protein Ag3904
• Vollständiger Name: cystinosis, nephropathic
• Berechnetes Molekulargewicht: 400aa,45 kDa; 367aa,42 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC032850
• Gene symbol: CTNS
• Gene ID (NCBI): 1497
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CTNS or cystinosin is a lysosomal membrane protein which comprises with seven transmembrane domains, a 128 amino acid N-terminal region bearing seven N-glycosylation sites and a cytosolic C-terminal GYDQL sorting motif. It functions as a H+-driven cystine transporter responsible for cystine export from lysosomes. The mutation of CTNS gene cause an inherited disorder, cystinosis, characterized by defective lysosomal efflux of cystine. This antibody was raised against the N-terminal region of human cystinosin. Two isoforms of CTNS exist due to alternative splicing events and both of them can be detected through this antibody.

Publikationen

Species	Application	Title
	WB	Biochem Biophys Res Commun; Reduced phosphate transport in the renal proximal tubule cells in cystinosis is due to decreased expression of transporters rather than an energy defect.; Authors - Mary L Taub
human	WB	Biochem Biophys Res Commun; Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.; Authors - Jong-Jer Lee
mouse	IF	J Immunol Methods; Characterization of a rat monoclonal antibody raised against ferroptotic cells.; Authors - Sho Kobayashi