CTNNBL1 Polyklonaler Antikörper

CTNNBL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13665-1-AP

Synonyme

Beta catenin like protein 1, C20orf33, catenin, beta like 1, CTNNBL1, NAP, Nuclear associated protein, NYD SP19, P14L, PP8304

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 13665-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13665-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13665-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13665-1-AP (CTNNBL1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13665-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 13665-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13665-1-AP (CTNNBL1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausmilzgewebe, Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13665-1-AP bindet in WB, IHC, ELISA CTNNBL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTNNBL1 fusion protein Ag4578
• Vollständiger Name: catenin, beta like 1
• Berechnetes Molekulargewicht: 563 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC036739
• Gene symbol: CTNNBL1
• Gene ID (NCBI): 56259
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTNNBL1, also named as C20orf33, belongs to the armadillo-containing protein family (PMID: 32484799). CTNNBL1 is a widely expressed nucleoprotein that associates with the pre-mRNA processing factor 19 (Prp19) complex of the spliceosome, interacting with its CDC5L component. CTNNBL1 can maintain the content and integrity of the Prp19 spliceosome complex by regulating the interaction between CWC15 and CDC5L (PMID: 28501461). CTNNBL1 has 4 isoforms with the molecular weights of 36, 43, 62 and 65 kDa, respectively. CTNNBL1 may play an important role in colorectal cancer and regulating AID function (PMID: 28501461, 32484799).

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Role of Glycated High Mobility Group Box-1 in Gastric Cancer.; Authors - Shingo Kishi
human	WB	J Transl Med; eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathway.; Authors - Liping Sun