CTNNA3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CTNNA3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 13974-1-AP

Synonyme

VR22, Catenin alpha-3, Catenin alpha 3, Cadherin-associated protein, Alpha T-catenin

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 13974-1-AP

  WB result of CTNNA3 antibody (13974-1-AP, 1:500) with si-Control and si-CTNNA3 transfected HEK293 cells.

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  WB analysis of mouse testis using 13974-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13974-1-AP (CTNNA3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13974-1-AP

  IP result of anti-CTNNA3 (IP:13974-1-AP, 5ug; Detection:13974-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13974-1-AP (CTNNA3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13974-1-AP (CTNNA3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 13974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13974-1-AP (CTNNA3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13974-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTNNA3 antibody 13974-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, humanes Plazenta-Gewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

13974-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CTNNA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTNNA3 fusion protein Ag5008
• Vollständiger Name: catenin (cadherin-associated protein), alpha 3
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC030646
• Gene symbol: CTNNA3
• Gene ID (NCBI): 29119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; Global landscape of HIV-human protein complexes.; Authors - Stefanie Jäger
mouse	IF	Cell Stem Cell; An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling.; Authors - Allison P Kann
mouse	IF	Circ Res; Alpha-Catenins Control Cardiomyocyte Proliferation by Regulating Yap Activity.; Authors - Jifen Li
human	WB	Nat Commun; Cell-cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas.; Authors - Fanjul-Fernández Miriam M ; KD Validated
mouse	IF	Elife; A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.; Authors - Jie Wang
mouse	IF,WB	Development; α-Catenin-dependent cytoskeletal tension controls Yap activity in the heart.; Authors - Alexia Vite ; KO Validated