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CTHRC1 Polyklonaler Antikörper
CTHRC1 Polyklonal Antikörper für IF, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 16534-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | A375-Zellen, HEK-293-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslungengewebe, Rattenlungengewebe |
Erfolgreiche IP | A375-Zellen |
Erfolgreiche Detektion in IF | A375-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunfluoreszenz (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 5 publications below |
WB | See 14 publications below |
IHC | See 10 publications below |
IF | See 5 publications below |
Produktinformation
16534-1-AP bindet in WB, IP, IHC, IF, ELISA CTHRC1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | CTHRC1 fusion protein Ag9812 |
Vollständiger Name | collagen triple helix repeat containing 1 |
Berechnetes Molekulargewicht | 243 aa, 26 kDa |
Beobachtetes Molekulargewicht | 30 kDa |
GenBank-Zugangsnummer | BC014245 |
Gene symbol | CTHRC1 |
Gene ID (NCBI) | 115908 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Collagen triple helix repeat-containing protein 1 (CTHRC1), also known as protein NMTC1, is a glycosylated secreted protein. CTHRC1 was identified as a novel molecular in injured and diseased arteries where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration (PMID: 15618538). It has been reported that CTHRC1 is a positive regulator of osteoblastic bone formation (PMID: 18779865). Besides, studies reveal that CTHRC1 expression is implicated in various malignancies, including hepatocellular carcinoma, gastric carcinoma, colon cancer, and lung cancer (PMID: 23922981; 24504172; 24746208; 25139095).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for CTHRC1 antibody 16534-1-AP | Protokoll herunterladen |
IF protocol for CTHRC1 antibody 16534-1-AP | Protokoll herunterladen |
IP protocol for CTHRC1 antibody 16534-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Theranostics Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer. | ||
Respir Res Pirfenidone attenuates lung fibrotic fibroblast responses to transforming growth factor-β1. | ||
J Cell Sci miR-9 inhibits Schwann cell migration by targeting CTHRC1 following sciatic nerve injury.
| ||
Inflammation Collagen Triple Helix Repeat Containing 1 Deficiency Protects Against Airway Remodeling and Inflammation in Asthma Models In Vivo and In Vitro
| ||
Int J Biol Sci NEDD4L-induced β-catenin ubiquitination suppresses the formation and progression of interstitial pulmonary fibrosis via inhibiting the CTHRC1/HIF-1α axis. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Valeria (Verified Customer) (11-11-2020) | Fixative: 4% paraformaldehydeAntigen retrieval step: NoPermeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)Protein blocking: 10% NGS diluted in TBSTEndogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, Goat anti-Rabbit isotype IgG, 1:100
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