CTBS Polyklonaler Antikörper

CTBS Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12599-1-AP

Synonyme

EC:3.2.1.-, Di-N-acetylchitobiase, CTB

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of SMMC-7721 using 12599-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of MCF-7 using 12599-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12599-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12599-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12599-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12599-1-AP (CTBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12599-1-AP (CTBS Antibody) at dilution of 1:50.

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12599-1-AP (CTBS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 12599-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12599-1-AP (CTBS antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12599-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTBS antibody 12599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maus-Kolongewebe, MCF-7-Zellen, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12599-1-AP bindet in WB, IHC, IF/ICC, ELISA CTBS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTBS fusion protein Ag3228
• Vollständiger Name: chitobiase, di-N-acetyl-
• Berechnetes Molekulargewicht: 12 kDa, 43 kDa
• Beobachtetes Molekulargewicht: 44-50 kDa
• GenBank-Zugangsnummer: BC024007
• Gene symbol: CTBS
• Gene ID (NCBI): 1486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTBS(Di-N-acetylchitobiase (chitobiase)) also named as CTB, is a lysosomal exoglycosidase that splits the GlcNAcβ-D-(l-4)GlcNAc chitobiose core of asparagine-linked glycoproteins. Chitobiase has been purified from human liver and kidney and rat liver. Both the human and rat enzymes are approximately 40-45 kDa and require a free reducing end GlcNAc for activity(PMID:1527079). This full length protein has a signal peptide and four glycosylation sites.