CTBP2 Polyklonaler Antikörper

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• KD/KO Validated

CTBP2 Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 10346-1-AP

Synonyme

C-terminal-binding protein 2

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Galerie der Validierungsdaten

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  WB analysis using 10346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10346-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10346-1-AP (CTBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10346-1-AP

  IP result of anti-CTBP2 (IP:10346-1-AP, 4ug; Detection:10346-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IF Staining of HeLa using 10346-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP2 antibody (10346-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10346-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CTBP2 (10346-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Produktinformation

10346-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA CTBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch, Mongolische Wüstenrennmaus (Gerbil)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTBP2 fusion protein Ag0352
• Vollständiger Name: C-terminal binding protein 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC002486
• Gene symbol: CTBP2
• Gene ID (NCBI): 1488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-Terminal binding protein 2 (CTBP2) is a transcriptional repressor. It contains a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. This protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggested that this protein is involved in transcriptional repression. CTBP2 is expressed in all tissues tested, with a higher level of expression in the heart, skeletal muscle, and pancreas. The gene of CTBP2 is mapped to human chromosome 21q21.3.

Publikationen

Species	Application	Title
zebrafish	IF	PLoS Biol; Hair cell identity establishes labeled lines of directional mechanosensation.; Authors - Marta Lozano-Ortega
gerbil	IF	J Neurosci; Tonotopic variation in the calcium dependence of neurotransmitter release and vesicle pool replenishment at mammalian auditory ribbon synapses.; Authors - Johnson Stuart L SL
mouse	IF	Neural Plast; Specific Influences of Early Acoustic Environments on Cochlear Hair Cells in Postnatal Mice.; Authors - Aoshuang Chang
mouse	WB	Proteome Sci; Proteomic analysis of neonatal mouse hearts shows PKA functions as a cardiomyocyte replication regulator; Authors - Lizhi Hu
zebrafish	IF	Genet Mol Res; MicroRNA-200 family members are weakly expressed in the neurosensory epithelia of the developing zebrafish (Danio rerio) inner ear.; Authors - J T Du
human	WB,IHC,IF	J Clin Invest; The CTBP2-PCIF1 complex regulates m6Am modification of mRNA in head and neck squamous cell carcinoma; Authors - Kang Li ; KO Validated