CSNK2B Polyklonaler Antikörper

CSNK2B Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 22418-1-AP

Synonyme

Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

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Galerie der Validierungsdaten

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  WB analysis using 22418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22418-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HeLa using 22418-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Casein Kinase 2 beta antibody (22418-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Schilddrüsengewebe, Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

22418-1-AP bindet in WB, IHC, FC, ELISA CSNK2B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSNK2B fusion protein Ag18134
• Vollständiger Name: casein kinase 2, beta polypeptide
• Berechnetes Molekulargewicht: 215 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC112017
• Gene symbol: CSNK2B
• Gene ID (NCBI): 1460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

Publikationen

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
human	WB	Cancer Metab; Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1; Authors - Hiroki Okanishi