Casein Kinase 1 Epsilon Polyklonaler Antikörper

Casein Kinase 1 Epsilon Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 11230-1-AP

Synonyme

Casein Kinase 1 epsilon, casein kinase 1, epsilon, CKI epsilon, CKIe, CSNK1E, HCKIE

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Galerie der Validierungsdaten

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  WB analysis of A431 using 11230-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11230-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11230-1-AP (Casein Kinase 1 epsilon antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 11230-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11230-1-AP (Casein Kinase 1 epsilon antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Eierstockgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

11230-1-AP bindet in WB, IHC, ELISA Casein Kinase 1 Epsilon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Casein Kinase 1 Epsilon fusion protein Ag1742
• Vollständiger Name: casein kinase 1, epsilon
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: BC006490
• Gene symbol: CK1 epsilon
• Gene ID (NCBI): 1454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK1E(Casein kinase I isoform epsilon) is also named as CKIe, CKI-epsilon and belongs to the casein kinase I subfamily. It plays a significant role in delaying the negative feedback signal within the transcription-translation-based autoregulatory loop that composes the core of the circadian mechanism and makes an ideal target for pharmaceutical compounds influencing circadian rhythms, sleep, and jet lag, as well as other physiologic and metabolic processes under circadian regulation(PMID:10775102). It can be autophosphorylated(PMID:19690332).

Publikationen

Species	Application	Title
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H