CSK Polyklonaler Antikörper

CSK Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17720-1-AP

Synonyme

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

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Galerie der Validierungsdaten

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  WB analysis using 17720-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 17720-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17720-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17720-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17720-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17720-1-AP (CSK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 17720-1-AP

  IP Result of anti-CSK (IP:17720-1-AP, 4ug; Detection:17720-1-AP 1:800) with HL-60 cells lysate 1000ug.

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  IHC staining of human lung using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17720-1-AP (CSK antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17720-1-AP (CSK antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of SH-SY5Y using 17720-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 17720-1-AP (CSK antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Rattenlungengewebe
• Erfolgreiche IP: HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

17720-1-AP bindet in WB, IP, IHC, IF, ELISA CSK und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSK fusion protein Ag12103
• Vollständiger Name: c-src tyrosine kinase
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC106073
• Gene symbol: CSK
• Gene ID (NCBI): 1445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

Publikationen

Species	Application	Title
human	IHC	Cancer Res; miR-31 Displays Subtype Specificity in Lung Cancer.; Authors - Mackenzie L Davenport
human	WB	Cell Chem Biol; A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes.; Authors - Benika J Pinch
human	WB	J Immunol; Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.; Authors - Hagberg Niklas N
human	IP	Neoplasia; SUMOylation of Csk Negatively Modulates its Tumor Suppressor Function.; Authors - Nan Cui