CSDA Polyklonaler Antikörper

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• KD/KO Validated

CSDA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27785-1-AP

Synonyme

cold shock domain protein A, Cold shock domain-containing protein A, DBPA, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB

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  WB analysis using 27785-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of KYSE-30 using 27785-1-AP

  WB result of CSDA antibody (27785-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells.

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  IHC staining of mouse heart using 27785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 27785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 27785-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSDA antibody (27785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27785-1-AP bindet in WB, IHC, IF/ICC, ELISA CSDA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSDA fusion protein Ag26839
• Vollständiger Name: cold shock domain protein A
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 60-68 kDa
• GenBank-Zugangsnummer: BC008801
• Gene symbol: CSDA
• Gene ID (NCBI): 8531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis; Authors - Lin-Yun Chen