Mu Crystallin Polyklonaler Antikörper

Mu Crystallin Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12495-1-AP

Synonyme

CRYM, NADP-regulated thyroid-hormone-binding protein, Ketimine reductase mu-crystallin, EC:1.5.1.25, DFNA40

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 12495-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12495-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12495-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12495-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12495-1-AP (mu Crystallin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12495-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12495-1-AP (mu Crystallin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human gliomas using 12495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using Mu Crystallin antibody (12495-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 12495-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human Mu Crystallin (12495-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, humanes Herzgewebe, humanes Nierengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Gliomgewebe
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12495-1-AP bindet in WB, IHC, IF-P, FC (Intra), ELISA Mu Crystallin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Mu Crystallin fusion protein Ag3161
• Vollständiger Name: crystallin, mu
• Berechnetes Molekulargewicht: 314 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC018061
• Gene symbol: Mu Crystallin
• Gene ID (NCBI): 1428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mu Crystallin(thiomorpholine-carboxylate dehydrogenase) is also named as THBP, CRYM, ketimine reductase and belongs to the ornithine cyclodeaminase family. This protein catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). It is also involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptor.

Publikationen

Species	Application	Title
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
rat	WB	Endocr J; Follicular thyroglobulin enhances gene expression necessary for thyroid hormone secretion.; Authors - Yuko Ishido
rat	WB,IHC,IF	Neuropathology; Expression of CRYM in different rat organs during development and its decreased expression in degenerating pyramidal tracts in amyotrophic lateral sclerosis.; Authors - Reiji Hommyo

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C