Cryptochrome 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cryptochrome 1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (2)

Kat-Nr. : 13474-1-AP

Synonyme

CRY1, Cryptochrome-1, CRY 1

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Galerie der Validierungsdaten

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  WB analysis using 13474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 13474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13474-1-AP

  Immunofluorescent analysis of Hela cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of MCF-7 using 13474-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 13474-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, Rattenlebergewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 27 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

13474-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA Cryptochrome 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cryptochrome 1 fusion protein Ag4270
• Vollständiger Name: cryptochrome 1 (photolyase-like)
• Berechnetes Molekulargewicht: 586 aa, 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC030519
• Gene symbol: CRY1
• Gene ID (NCBI): 1407
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

Publikationen

Species	Application	Title
	WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
human	IF	Theranostics; Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.; Authors - Mark A Naven
human	WB	EMBO Rep; Circadian networks in human embryonic stem cell-derived cardiomyocytes.; Authors - Pieterjan Dierickx
human	WB	Stem Cell Reports; SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.; Authors - Bastiaan C Du Pré
human,mouse	WB,CoIP	Biochem Pharmacol; CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression of E4BP4; Authors - Luomin Lin ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-11-2023)

I have used this for Human cardiomyocytes cell line and mice skin, and liver tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

FH

Iru (Verified Customer) (06-26-2020)

It works fine in 1:1000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells