CRNN Polyklonaler Antikörper

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• KD/KO Validated

CRNN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11799-1-AP

Synonyme

58 kDa heat shock protein, C1orf10, cornulin, CRNN, DRC1, PDRC1, SEP53, Tumor related protein

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 11799-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11799-1-AP (CRNN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11799-1-AP (CRNN Antibody) at dilution of 1:50.

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  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11799-1-AP (CRNN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human oesophagus using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200.

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 11799-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 11799-1-AP (CRNN Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Hautgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 9 publications below
• IF: See 2 publications below

Produktinformation

11799-1-AP bindet in WB, IHC, IF, ELISA CRNN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRNN fusion protein Ag2374
• Vollständiger Name: cornulin
• Berechnetes Molekulargewicht: 495 aa, 54 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 58 kDa
• GenBank-Zugangsnummer: BC030807
• Gene symbol: CRNN
• Gene ID (NCBI): 49860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cornulin, also known as C1orf10, is a member of the "fused gene" family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. It may play a role in the mucosal/epithelial immune response and epidermal differentiation. Specific to squamous epithelia cells, cornulin is expressed in esophagus, primary keratinocytes, scalp skin, foreskin, cervix, larynx, and fetal bladder. It may be a marker of late epidermal differentiation, and is down-regulated in eczema.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	J Invest Dermatol; Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways.; Authors - Changji Li ; KD Validated
human	WB,IHC	J Proteomics; Plectin promotes migration and invasion of cancer cells and is a novel prognostic marker for head and neck squamous cell carcinoma.; Authors - Katada Koji K
human	IHC	Clin Oral Investig; Downregulation of CRNN gene and genomic instability at 1q21.3 in oral squamous cell carcinoma.; Authors - Iman Salahshourifar
human	IHC	J Thorac Cardiovasc Surg; Loss of CRNN expression is associated with advanced tumor stage and poor survival in patients with esophageal squamous cell carcinoma.; Authors - Hsu Po-Kuei PK
human	IHC	PLoS One; Expression of Ki-67, Cornulin and ISG15 in non-involved mucosal surgical margins as predictive markers for relapse in oral squamous cell carcinoma (OSCC); Authors - Padmanabha Kumar Govindaraj
human	IHC	Ecancermedicalscience; Expression of cornulin in tongue squamous cell carcinoma.; Authors - Saira Saleem