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CRK Monoklonaler Antikörper

CRK Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

ELISA

Konjugation

Biotin

CloneNo.

2B7E1

Kat-Nr. : Biotin-67035

Synonyme

Adapter molecule crk, CRK, CRKII, p38, Proto oncogene c Crk

Galerie der Validierungsdaten

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  • ELISA experiment of Ag28793 using Biotin-conjugated CRK Monoclonal antibody (Biotin-67035)

    ELISA experiment of Ag28793 using Biotin-67035

    Biotin-67035 tested by ELISA. Ag28793(CRK 41-304 aa)was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of Biotin-67035 (start dilution 1:500. Bound antibodies were detected with Streptavidin Poly-HRP(1:5000)followed by incubation with HRP Substrate,terminated with 2M H2SO4,then measuring the resulting absorbance at 450 nm.

Geprüfte Anwendungen

Erfolgreiche Detektion in ELISAAg28793

Empfohlene Verdünnung

AnwendungVerdünnung
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-67035 bindet in ELISA CRK und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CRK fusion protein Ag28793
Vollständiger Name v-crk sarcoma virus CT10 oncogene homolog (avian)
Berechnetes Molekulargewicht 34 kDa
Beobachtetes Molekulargewicht 34 kDa
GenBank-ZugangsnummerBC008506
Gene symbol CRK
Gene ID (NCBI) 1398
Konjugation Biotin
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRK is a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. CRK has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation.