CRIPT Polyklonaler Antikörper

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• KD/KO Validated

CRIPT Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11211-1-AP

Synonyme

HSPC139, Cysteine-rich PDZ-binding protein, Cysteine-rich interactor of PDZ3, Cysteine-rich interactor of PDZ three

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Galerie der Validierungsdaten

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  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11211-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11211-1-AP (CRIPT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 11211-1-AP

  IP result of anti-CRIPT (IP:11211-1-AP, 4ug; Detection:11211-1-AP 1:500) with L02 cells lysate 1800ug.

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  IF Staining of L02 using 11211-1-AP

  Immunofluorescent analysis of L02 cells using 11211-1-AP (CRIPT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11211-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA CRIPT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRIPT fusion protein Ag1708
• Vollständiger Name: cysteine-rich PDZ-binding protein
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC018653
• Gene symbol: CRIPT
• Gene ID (NCBI): 9419
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains

Publikationen

Species	Application	Title
rat	WB,CoIP	Mol Neurobiol; Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.; Authors - Anton Omelchenko ; KD Validated
rat	WB,IP	eNeuro; SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo.; Authors - Lei Zhang