CREG1 Polyklonaler Antikörper

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• KD/KO Validated

CREG1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12220-1-AP

Synonyme

CREG, CREG1, Protein CREG1

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Galerie der Validierungsdaten

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  WB analysis using 12220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12220-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12220-1-AP bindet in WB, IHC, ELISA, IF CREG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREG1 fusion protein Ag2901
• Vollständiger Name: cellular repressor of E1A-stimulated genes 1
• Berechnetes Molekulargewicht: 220 aa, 24 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC008628
• Gene symbol: CREG1
• Gene ID (NCBI): 8804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).

Publikationen

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
mouse	WB	Int J Mol Sci; TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle.; Authors - Haruna Fujiyoshi
human	WB	Carcinogenesis; PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1; Authors - Ming Xiong
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang
mouse	WB,IHC	J Biochem; CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.; Authors - Tatsuya Kusudo ; KD Validated
		FASEB J; CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.; Authors - Michihiro Hashimoto