RAIDD Polyklonaler Antikörper

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• KD/KO Validated

RAIDD Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10401-1-AP

Synonyme

CRADD, RAIDD

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 10401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10401-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10401-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10401-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10401-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10401-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10401-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10401-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10401-1-AP (RAIDD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10401-1-AP

  IP result of anti-RAIDD (IP:10401-1-AP, 3ug; Detection:10401-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 10401-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10401-1-AP (RAIDD antibody) at dilution of 1:100 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, Mausherzgewebe, Maushodengewebe, Mauslebergewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below

Produktinformation

10401-1-AP bindet in WB, IP, IHC, ELISA RAIDD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAIDD fusion protein Ag0630
• Vollständiger Name: CASP2 and RIPK1 domain containing adaptor with death domain
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC017042
• Gene symbol: CRADD
• Gene ID (NCBI): 8738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.

Publikationen

Species	Application	Title
mouse	WB	Dev Cell; E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.; Authors - Valentina C Sladky ; KD Validated
mouse	WB	EMBO Rep; PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis.; Authors - Valentina C Sladky
mouse	WB	J Immunol; Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes.; Authors - Lin Qing Q ; KO Validated
human, mouse	WB	J Biol Chem; The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli.; Authors - Huan Qiao
mouse	WB	Cell Metab; PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis; Authors - Ju Youn Kim ; KO Validated