CPSF6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CPSF6 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 15489-1-AP

Synonyme

CFIM, CFIM68, CPSF 68 kDa subunit, CPSF6, HPBRII 4, HPBRII 7, Protein HPBRII 4/7

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15489-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15489-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15489-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15489-1-AP

  IP Result of anti-CPSF6 (IP:15489-1-AP, 4ug; Detection:15489-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15489-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SW480 using 15489-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CPSF6 antibody (15489-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, MDA-MB-231-Zellen, PC-3-Zellen, SW480-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 9 publications below

Produktinformation

15489-1-AP bindet in WB, IP, IHC, IF, ELISA CPSF6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF6 fusion protein Ag7852
• Vollständiger Name: cleavage and polyadenylation specific factor 6, 68kDa
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 55-68 kDa
• GenBank-Zugangsnummer: BC000714
• Gene symbol: CPSF6
• Gene ID (NCBI): 11052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

Publikationen

Species	Application	Title
human	WB	Mol Cell; A Quantitative Genetic Interaction Map of HIV Infection.; Authors - David E Gordon
human	WB	PLoS Pathog; In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.; Authors - Matthew S Henning
human	WB	Elife; Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.; Authors - Melissa Kane
human	WB	J Virol; Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.; Authors - Augustin Penda Twizerimana
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	J Virol; Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.; Authors - Akatsuki Saito ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, SH-SY5Y cells