CPSF3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CPSF3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 11609-1-AP

Synonyme

mRNA 3'-end-processing endonuclease CPSF-73, EC:3.1.27.-, CPSF73, CPSF 73 kDa subunit, CPSF 73

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Galerie der Validierungsdaten

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  WB analysis using 11609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11609-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11609-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

• 

  IHC staining of mouse kidney using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11609-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Plazenta-Gewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below

Produktinformation

11609-1-AP bindet in WB, IHC, IP, ELISA CPSF3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF3 fusion protein Ag2173
• Vollständiger Name: cleavage and polyadenylation specific factor 3, 73kDa
• Berechnetes Molekulargewicht: 684 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC020211
• Gene symbol: CPSF3
• Gene ID (NCBI): 51692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.; Authors - Kaili Luo ; KD Validated
human	WB	Cancers (Basel); CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma; Authors - Ying Huang
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
pig	WB	Cell Mol Life Sci; Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs; Authors - Xiaolong Yuan
human	WB	Cell Chem Biol; Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3; Authors - Ye Tao
human	WB	Cell Discov; RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation; Authors - Peng Lin ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (06-12-2024)

Works perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cell line

CPSF3 Antibody Western Blot validation (1:1000 dilution) in HeLa cell line (Cat no:11609-1-AP)