CPEB3 Polyklonaler Antikörper

CPEB3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12669-1-AP

Synonyme

hCPEB 3, Cytoplasmic polyadenylation element-binding protein 3, CPE-BP3, CPE-binding protein 3, CPE BP3

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Galerie der Validierungsdaten

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  WB analysis using 12669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12669-1-AP (CPEB3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12669-1-AP (CPEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12669-1-AP (CPEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12669-1-AP (CPEB3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12669-1-AP bindet in WB, IHC, IF-P, ELISA CPEB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPEB3 fusion protein Ag3351
• Vollständiger Name: cytoplasmic polyadenylation element binding protein 3
• Berechnetes Molekulargewicht: 697 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76-80 kDa
• GenBank-Zugangsnummer: BC036444
• Gene symbol: CPEB3
• Gene ID (NCBI): 22849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPEB3, the cytoplasmic polyadenylation element-binding protein 3, contains a Q/N-rich domain at its N-termina which is required for the aggregation of CPEB3. The expression of CPEB3 is downregulated in cervical cancer and hepatocellular carcinoma compared with normal tissue. It has been reported that the aggregation and activity of CPEB3 is controlled by SUMOylation. CPEB3 is SUMOylated in hippocampal neurons in the basal state while converted into an active form which is associated with a decrease of SUMOylation and an increase of aggregation. CPEB3 is expressed in cytoplasma in unstimulated neurons but translocates to the nucleus following neuronal stimulation.

Publikationen

Species	Application	Title
human	WB	Pharmacology; MiR-224 Executes a Tumor Accelerative Role during Hepatocellular Carcinoma Malignancy by Targeting Cytoplasmic Polyadenylation Element-Binding Protein 3.; Authors - Ke Miao