Carboxypeptidase E Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Carboxypeptidase E Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 13710-1-AP

Synonyme

Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, CPE, Carboxypeptidase H

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13710-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13710-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13710-1-AP

  IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug.

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  IHC staining of human pituitary using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pituitary using 13710-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 13710-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13710-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hypophysengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

13710-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Carboxypeptidase E und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase E fusion protein Ag4647
• Vollständiger Name: carboxypeptidase E
• Berechnetes Molekulargewicht: 476 aa, 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 52 kDa
• GenBank-Zugangsnummer: BC033866
• Gene symbol: Carboxypeptidase E
• Gene ID (NCBI): 1363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.

Publikationen

Species	Application	Title
rat	WB	Cell Metab; Reductive TCA cycle metabolism fuels glutamine- and glucose-stimulated insulin secretion.; Authors - Guo-Fang Zhang
mouse	WB	Diabetes; Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.; Authors - Seokwon Jo
		Am J Physiol Endocrinol Metab; Loss of the Golgi-localized v-ATPase subunit does not alter insulin granule formation or pancreatic islet β-cell function; Authors - Cierra K Boyer
human, mouse	IHC	Brain Pathol; Secretory sorting receptors carboxypeptidase E and secretogranin III in amyloid β-associated neural degeneration in Alzheimer's disease.; Authors - Virginia Plá
human	WB,IHC	Front Genet; Development and Validation of a Novel Gene Signature for Predicting the Prognosis by Identifying m5C Modification Subtypes of Cervical Cancer.; Authors - Jing Yu ; KD Validated
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li