COX7A1 Polyklonaler Antikörper

COX7A1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11413-1-AP

Synonyme

COX7A, COX7A1, COX7AH, COX7AM

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse heart using 11413-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11413-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11413-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11413-1-AP (COX7A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11413-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11413-1-AP (COX7A1 antibody) at dilution of 1:100 (under 10x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11413-1-AP bindet in WB, IHC, IF, ELISA COX7A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX7A1 fusion protein Ag1981
• Vollständiger Name: cytochrome c oxidase subunit VIIa polypeptide 1 (muscle)
• Berechnetes Molekulargewicht: 79 aa, 9 kDa
• Beobachtetes Molekulargewicht: 7-9 kDa
• GenBank-Zugangsnummer: BC002757
• Gene symbol: COX7A1
• Gene ID (NCBI): 1346
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome c oxidase(COX) catalyzes the transfer of electrons from reduced cytochrome c to molecular oxygen. Subunit VIIa of mammalian COX exists in at least 2 isoforms, liver and muscle. COX7A1(Cytochrome c oxidase subunit 7A1, mitochondrial) is also named as COX7AH, cytochrome c oxidase subunit VIIa-H, cytochrome c oxidase subunit VIIa-M and is the muscle isoform of COX subunit VIIa. Northern-blot analysis of primate tissues demonstrated that COXVIIa-M mRNA is present only in muscle tissues(PMID:1327965).

Publikationen

Species	Application	Title
rat	IHC	Cereb Cortex; New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.; Authors - Ben Shabat Moti
human	WB	Lab Invest; Overexpression of COX7A1 Promotes the Resistance of Gastric Cancer to Oxaliplatin and Weakens the Efficacy of Immunotherapy; Authors - Si-Yu Wang
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
human	WB, IF	Int J Biochem Cell Biol; Investigating the role of the physiological isoform switch of cytochrome c oxidase subunits in reversible mitochondrial disease.; Authors - Veronika Boczonadi
mouse	WB	Biochim Biophys Acta Mol Cell Res; Blockade of angiotensin II modulates insulin-like growth factor 1-mediated skeletal muscle homeostasis in experimental steatohepatitis; Authors - Misako Tanaka