COX6B1 Polyklonaler Antikörper

COX6B1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11425-1-AP

Synonyme

COX6B, COX VIb-1, COX VIb 1

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Galerie der Validierungsdaten

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  WB analysis using 11425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 11425-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11425-1-AP (COX6B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11425-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11425-1-AP (COX6B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11425-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COX6B1 antibody (11425-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Gliomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

11425-1-AP bindet in WB, IHC, IF/ICC, ELISA COX6B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX6B1 fusion protein Ag1984
• Vollständiger Name: cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous)
• Berechnetes Molekulargewicht: 80 aa, 10 kDa
• Beobachtetes Molekulargewicht: 10-13 kDa
• GenBank-Zugangsnummer: BC001015
• Gene symbol: COX6B1
• Gene ID (NCBI): 1340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

Publikationen

Species	Application	Title
human	WB	J Cell Biol; PDGFRβ translocates to the nucleus and regulates chromatin remodeling via TATA element-modifying factor 1.; Authors - Natalia Papadopoulos
human	WB	Hum Reprod; Lysine glutarylation in human sperm is associated with progressive motility.; Authors - Yi-Min Cheng
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng
human	WB	J Biol Chem; The cellular stress proteins CHCHD10 and MNRR1 (CHCHD2): Partners in mitochondrial and nuclear function and dysfunction.; Authors - Neeraja Purandare
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
rat	WB	Mol Med Rep; Overexpression of COX6B1 protects against I/R‑induced neuronal injury in rat hippocampal neurons.; Authors - Shan Yang