CORO2A Polyklonaler Antikörper

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• KD/KO Validated

CORO2A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28883-1-AP

Synonyme

CLIPINB, CORO2A, Coronin 2A, DKFZp686G19226, IR10, WD repeat containing protein 2, WDR2

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Galerie der Validierungsdaten

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  WB analysis using 28883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28883-1-AP (CORO2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 28883-1-AP

  WB result of CORO2A antibody (28883-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CORO2A transfected Jurkat cells.

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  IHC staining of human skin cancer using 28883-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28883-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 28883-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 28883-1-AP (CORO2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 28883-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28883-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 28883-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28883-1-AP (CORO2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28883-1-AP bindet in WB, IHC, ELISA CORO2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CORO2A fusion protein Ag29696
• Vollständiger Name: coronin, actin binding protein, 2A
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000010
• Gene symbol: CORO2A
• Gene ID (NCBI): 7464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CORO2A (Coronin 2A), also named as CLIPINB, is a member of the 'short' coronin subfamily containing a single WD40-repeat domain. It has been shown that the expression of CORO2A is up-regulated in human colon cancer. CORO2A can interact with MAPK14 and PRMT5. The calculated MW of CORO2A is 60 kDa, while 28883-1-AP antibody detects 48 kDa protein which is similar to the paper published. (PMID: 26373535)