COQ6 Polyklonaler Antikörper

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• KD/KO Validated

COQ6 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 12481-1-AP

Synonyme

CGI 10, COQ6

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Galerie der Validierungsdaten

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  WB analysis using 12481-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12481-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 12481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 12481-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12481-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12481-1-AP (COQ6 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12481-1-AP bindet in WB, IF, IHC, ELISA COQ6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COQ6 fusion protein Ag3164
• Vollständiger Name: coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
• Berechnetes Molekulargewicht: 468 aa, 51 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC014181
• Gene symbol: COQ6
• Gene ID (NCBI): 51004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB, IF	Aging Cell; Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging.; Authors - Assaf Ben-Meir
human	WB	Oxid Med Cell Longev; Vanillic Acid Restores Coenzyme Q Biosynthesis and ATP Production in Human Cells Lacking COQ6.; Authors - Manuel J Acosta Lopez ; KO Validated
human	WB	J Assist Reprod Genet; PMID: 26552664; Authors - Yaakov Bentov
human	IHC	Chin Med J (Engl); New Mutation of Coenzyme Q10 Monooxygenase 6 Causing Podocyte Injury in a Focal Segmental Glomerulosclerosis Patient.; Authors - Cheng-Cheng Song
mouse	IF	Antioxidants (Basel); Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model.; Authors - Assaf Ben-Meir