COPS8/COP9 Polyklonaler Antikörper

COPS8/COP9 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10089-2-AP

Synonyme

COP9, COP9 homolog, COPS8, COPS8/COP9, CSN8, hCOP9, SGN8, Signalosome subunit 8

---

Galerie der Validierungsdaten

• 

  WB analysis using 10089-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10089-2-PBS

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10089-2-AP

  IP result of anti-COPS8/COP9 (IP:10089-2-AP, 4ug; Detection:10089-2-AP 1:500) with mouse brain tissue lysate 3200 ug.

• 

  IHC staining of human colon cancer using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10089-2-AP (COPS8/COP9 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10089-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10089-2-AP (COPS8/COP9 antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10089-2-AP bindet in WB, IP, IHC, ELISA COPS8/COP9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPS8/COP9 fusion protein Ag0143
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC003090
• Gene symbol: COPS8
• Gene ID (NCBI): 10920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.

Publikationen

Species	Application	Title
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	WB	Biochem Biophys Res Commun; Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex.; Authors - Xu Gui-Ping GP
mouse	WB	Neuropsychiatr Dis Treat; COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.; Authors - En Liang