CSN2 Monoklonaler Antikörper

CSN2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

CloneNo.

2H10E6

Kat-Nr. : 66435-1-Ig

Synonyme

ALIEN, Alien homolog, COPS2, CSN2, SGN2, Signalosome subunit 2, TR interacting protein 15, TRIP 15, TRIP15

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 66435-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66435-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66435-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66435-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66435-1-Ig (CSN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66435-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (CSN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66435-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66435-1-Ig (COPS2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human ovary tumor using 66435-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CSN2 antibody (66435-1-Ig, Clone: 2H10E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen, RAW 264.7-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Ovarialkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66435-1-Ig bindet in WB, IHC, IF CSN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSN2 fusion protein Ag1415
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC012629
• Gene symbol: COPS2
• Gene ID (NCBI): 9318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.