CSN2 Polyklonaler Antikörper

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• KD/KO Validated

CSN2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 10969-2-AP

Synonyme

COPS2, JAB1-containing signalosome subunit 2, COPS2/TRIP15, COPS 2, Alien homolog

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Galerie der Validierungsdaten

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  WB analysis using 10969-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10969-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10969-2-AP

  WB result of CSN2 antibody (10969-2-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSN2 transfected HEK-293 cells.

• 

  WB analysis of mouse brain using 10969-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10969-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10969-2-AP (CSN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 10969-2-AP

  IP result of anti-CSN2 (IP:10969-2-AP, 3ug; Detection:10969-2-AP 1:500) with NIH/3T3 cells lysate 2500ug.

• 

  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10969-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10969-2-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10969-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CSN2 antibody (10969-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HEK-293-Zellen, Maushirngewebe, Mausnierengewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10969-2-AP bindet in WB, IHC, IF/ICC, IP, ELISA CSN2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSN2 fusion protein Ag1415
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC012629
• Gene symbol: COPS2
• Gene ID (NCBI): 9318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPS2 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. (refer to UniProt) Catalog#10969-2-AP is a rabbit polyclonal antibody raised against the full-length of human COPS2. The MW of this protein is 52 kDa, and this antibody specially recognises the 52 kDa protein.

Publikationen

Species	Application	Title
human	WB	Mol Cell; Glucose-induced CRL4COP1-p53 axis amplifies glycometabolism to drive tumorigenesis; Authors - Yang Su
mouse	WB	Nat Commun; IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.; Authors - Hong Lin ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.; Authors - Feng Rao
human	WB	Proc Natl Acad Sci U S A; Inositol hexakisphosphate (IP6) generated by IP5K mediates cullin-COP9 signalosome interactions and CRL function.; Authors - Paul C Scherer
human	WB	Proc Natl Acad Sci U S A; Basis for metabolite-dependent Cullin-RING ligase deneddylation by the COP9 signalosome.; Authors - Hong Lin
human	WB	iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Resha (Verified Customer) (11-01-2023)

In 20ug of total protein, 1:1000 dilution of anti-COPS2 antibody gave clear band of 52kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells