COLEC11 Polyklonaler Antikörper

COLEC11 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (2)

Kat-Nr. : 15269-1-AP

Synonyme

CL K1, CL K1 I, CL K1 II, CL K1 IIa, CL K1 IIb, CLK1, COLEC11, Collectin 11, Collectin kidney protein 1, collectin sub family member 11

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 15269-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15269-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 15269-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 15269-1-AP (COLEC11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15269-1-AP

  IP Result of anti-COLEC11 (IP:15269-1-AP, 4ug; Detection:15269-1-AP 1:500) with mouse liver tissue lysate 6400ug.

• 

  IHC staining of mouse liver using 15269-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15269-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15269-1-AP (COLEC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of ATCD5 cells using 15269-1-AP

  IF result (confocal image) of CL-K1 in ATCD5 cell show Golgi localization from Prof. Philip L. Beales.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, human plasma
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: ATCD5-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

15269-1-AP bindet in WB, IP, IHC, IF, FC, ELISA COLEC11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COLEC11 fusion protein Ag7374
• Vollständiger Name: collectin sub-family member 11
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC000078
• Gene symbol: COLEC11
• Gene ID (NCBI): 78989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COLEC11, also named as CL-K1 and Collectin-11, belongs to the COLEC10/COLEC11 family. It is a lectin that binds to various sugars: fucose > mannose. It does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. COLEC11 binds to LPS. COLEC11 and MASP1 are two genes in the lectin complement pathway, they are mutated in 3MC syndrome, implicating this diverse inflammation-chemotaxis cascade in the etiology of human developmental disorders. COLEC11 serves as a guidance cue for neural crest cell migration.(PMID:21258343)

Publikationen

Species	Application	Title
mouse	IHC,IF	Nat Genet; Mutations in lectin complement pathway genes COLEC11 and MASP1 cause 3MC syndrome.; Authors - Rooryck Caroline C
human	WB,IF	Clin J Am Soc Nephrol; Collectin11 and Complement Activation in IgA Nephropathy.; Authors - Min Wei
mouse	WB	Front Immunol; Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1).; Authors - Rafael Bayarri-Olmos
human	WB	J Immunol; Heterocomplex Formation between MBL/Ficolin/CL-11-Associated Serine Protease-1 and -3 and MBL/Ficolin/CL-11-Associated Protein-1.; Authors - Anne Rosbjerg
human	FC	J Innate Immun; Collectin-11/MASP Complex Formation Triggers Activation of the Lectin Complement Pathway - The Fifth Lectin Pathway Initiation Complex.; Authors - Ma Ying Jie YJ
human	WB	Clin Proteomics; iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy.; Authors - Hongmei Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (08-28-2019)

Antibody works for identification of recombinant CL-11 well however multiple bands appears on cell lysates.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human PTECs

FH

mark (Verified Customer) (08-13-2019)

Consitent fro WB but binds non CL-11 in cell lysatesFor immunohistochemistry/immunoflourescence binds CL-11 consistently but also has off target effects (eg in CL-11 KO mice)

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for WB, 1:100 for Immuno
• Cell Tissue Type: mouse cell culture, mouse kidney, mouse embryos, human kidney

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