Coilin Polyklonaler Antikörper

Coilin Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 10967-1-AP

Synonyme

COIL, p80-coilin, p80 coilin, CLN80

---

Galerie der Validierungsdaten

• 

  WB analysis using 10967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10967-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10967-1-AP (Coilin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10967-1-AP

  IP result of anti-Coilin (IP:10967-1-AP, 4ug; Detection:10967-1-AP 1:10000) with HEK-293 cells lysate 1400 ug.

• 

  IHC staining of human prostate cancer using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10967-1-AP (Coilin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10967-1-AP (Coilin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10967-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Coilin antibody (10967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 10967-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Coilin (10967-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• CoIP: See 2 publications below

Produktinformation

10967-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA Coilin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Coilin fusion protein Ag1416
• Vollständiger Name: coilin
• Berechnetes Molekulargewicht: 576 aa, 63 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC010385
• Gene symbol: COIL
• Gene ID (NCBI): 8161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
mouse	WB,IHC,IF,CoIP	Clin Transl Med; HSF4/COIL complex-dependent R-loop mediates ultraviolet-induced inflammatory skin injury; Authors - Yi-Qian Feng
human	IF	PLoS Genet; Nuclear Fragile X Mental Retardation Protein Is localized to Cajal Bodies.; Authors - Dury Alain Y AY
human	IF	Elife; Characterisation of the biflavonoid hinokiflavone as a pre-mRNA splicing modulator that inhibits SENP.; Authors - Andrea Pawellek
human	WB,IF	Neurobiol Dis; UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.; Authors - Simona Rossi
human	WB,IF	Mol Brain; Long non-coding RNA lncC11orf54-1 modulates neuroinflammatory responses by activating NF-κB signaling during meningitic Escherichia coli infection; Authors - Bojie Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human satelllite muscle cells

Coilin Antibody Western Blot, validation (1:1000 dilution) in human satelllite muscle cells (Cat no:10967-1-AP)