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COG8 Polyklonaler Antikörper

COG8 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12661-1-AP

Synonyme

DOR1, COG complex subunit 8

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using COG8 Polyclonal antibody (12661-1-AP)

    WB analysis using 12661-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12661-1-AP (COG8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using COG8 Polyclonal antibody (12661-1-AP)

    IF Staining of A431 using 12661-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using COG8 antibody (12661-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Raji-Zellen
Erfolgreiche Detektion in IF/ICCA431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

12661-1-AP bindet in WB, IF/ICC, ELISA COG8 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen COG8 fusion protein Ag3341
Vollständiger Name component of oligomeric golgi complex 8
Berechnetes Molekulargewicht 612 aa, 68 kDa
Beobachtetes Molekulargewicht 68 kDa
GenBank-ZugangsnummerBC017492
Gene symbol COG8
Gene ID (NCBI) 84342
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The conserved oligomeric Golgi (COG) complex is an eight subunit (COG1-8) peripheral Golgi membrane hetero-oligomeric protein complex. The COG complex is organized into lobes A (COG2-4) and B (COG5-7) with COG1 and COG8 bridging these lobes and is thought to play a critical role in vesicle tethering processes (PMID: 17331980). Cog8 localizes exclusively in the Golgi apparatus and is involved in the retrograde Golgi transport of resident proteins responsible for sugar chain (glycan) biosynthesis (PMID: 17220172).

Protokolle

Produktspezifische Protokolle
WB protocol for COG8 antibody 12661-1-APProtokoll herunterladen
IF protocol for COG8 antibody 12661-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
View Article