Cannabinoid receptor 1 Polyklonaler Antikörper

Cannabinoid receptor 1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 17978-1-AP

Synonyme

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

---

Galerie der Validierungsdaten

• 

  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 17978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody (17978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 17978-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cannabinoid receptor 1 (17978-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

17978-1-AP bindet in WB, IHC, IF, FC, ELISA Cannabinoid receptor 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cannabinoid receptor 1 fusion protein Ag12371
• Vollständiger Name: cannabinoid receptor 1 (brain)
• Berechnetes Molekulargewicht: 472 aa, 53 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 66 kDa
• GenBank-Zugangsnummer: BC074812
• Gene symbol: CNR1
• Gene ID (NCBI): 1268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publikationen

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
mouse	WB	Chin Med; Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis; Authors - Jingze Yang
mouse	WB	Hum Mol Genet; BDNF overexpression prevents cognitive deficit elicited by adolescent cannabis exposure and host susceptibility interaction.; Authors - Hadar Segal-Gavish
rat	IHC	Phytomedicine; Endocannabinoid signalling/cannabinoid receptor 2 is involved in icariin-mediated protective effects against bleomycin-induced pulmonary fibrosis.; Authors - Wenjing Du
rat	WB	Food Funct; L-Theanine regulates glutamine metabolism and immune function by binding to cannabinoid receptor 1.; Authors - An Liu
mouse,rat	WB,IHC,IF	Int J Cardiol; Indoxyl sulfate upregulates the cannabinoid type 1 receptor gene via an ATF3/c-Jun complex-mediated signaling pathway in the model of uremic cardiomyopathy.; Authors - Yu-Juei Hsu

Verwandte Produkte

CNRIP1 Polyclonal antibody

16827-1-AP

FAAH Polyclonal antibody

17909-1-AP